Pandemic: In the Lab

Yes — lab actions must be done at research stations (p.3).

Yes — one cube removed by a Treat Disease action may be placed in either of the two sample dishes instead of being returned to the supply (p.4).

Process a Sample moves all cubes from one dish along a chosen arrow: the centrifuge keeps all cubes of one color, the separator keeps one of each color present, and moving to the growth dish doubles each cube using cubes from the supply; if you cannot place the required cubes into the growth dish because the supply is insufficient, the game ends and you lose (p.4).

No — a research line must be characterized (play a City card to set the cure color and move the vial) before any cubes may be moved onto its Sequence card (pp.3–4).

In the Lab challenge you discard 3 City cards of the cure color at a research station to Discover a Cure (the Scientist discards 2); the base-game requirement of 5 does not apply here (p.5).

No — to Test a Cure you must play a City card of the cure color onto the research line's test space and the Sequence card must already have at least one cube (the cube removed by the test is returned to the supply) (p.5).